Watch the intake: By evolving the N-terminal domain of pyrrolysyl-tRNA synthetase (PylRS), mutations that improve the genetic incorporation of N^ϵ-(tertbutoxycarbonyl)-l-lysine at the amber codon are identified. These mutations can be directly transferred to PylRS mutants for improved incorporation efficiency of their corresponding noncanonical amino acids.

中文翻译：

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进化的Pyrrolysyl-tRNA合成酶的N末端域，以改善非规范氨基酸的掺入。

观察摄入量：通过发展吡咯烷基-tRNA合成酶（PylRS）的N端结构域，鉴定出可改善琥珀色密码子上N ^ϵ-（叔丁氧羰基）-1-赖氨酸的遗传掺入的突变。可以将这些突变直接转移到PylRS突变体，以提高其相应的非规范氨基酸的掺入效率。