Persistent nanophosphors can remain luminescent after excitation ceases; thus, they offer a promising way to avoid background fluorescence interference in bioimaging. In this work, Zn₂GeO₄:Ga,Mn (ZGO:Ga,Mn) persistent luminescence nanoparticles were developed and they were employed for time-gated imaging of latent fingerprints (LFP). The nanoparticles were functionalized with a carboxyl group and utilized to label LFP through reacting with the amino group in the LFP. Results proved the potent ability of ZGO:Ga,Mn in eliminating background fluorescence to afford highly sensitive LFP imaging. Moreover, LFP aged for 60 days were successfully detected due to the presence of highly stable amino acids. After being functionalized with concanavalin A, the nanoparticles achieved visualization of glycoproteins in LFP. This strategy provides great versatility in LFP imaging and good potential in uncovering the chemical information within LFP, making it valuable in forensic investigations and medical diagnostics.

中文翻译：

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潜在指纹的时控成像和通过分子识别的蛋白质分泌物特异性可视化

持久的纳米磷光体在激发停止后仍可以保持发光。因此，它们提供了一种有前途的方法来避免生物成像中的背景荧光干扰。在这项工作中，Zn ₂ GeO ₄：Ga，Mn（ZGO：Ga，Mn）持久发光纳米粒子被开发出来，并被用于潜在指纹（LFP）的时间门控成像。纳米颗粒用羧基官能化，并通过与LFP中的氨基反应用于标记LFP。结果证明了ZGO：Ga，Mn消除背景荧光以提供高灵敏度LFP成像的强大能力。此外，由于存在高度稳定的氨基酸，可以成功检测到老化60天的LFP。在用伴刀豆球蛋白A功能化后，纳米颗粒实现了LFP中糖蛋白的可视化。该策略在LFP成像中具有很大的通用性，并且在发现LFP中的化学信息方面具有良好的潜力，使其在法医调查和医学诊断中具有重要的价值。