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Simultaneous Detection of Glutathione and Hydrogen Polysulfides from Different Emission Channels
Analytical Chemistry ( IF 7.4 ) Pub Date : 2017-11-15 00:00:00 , DOI: 10.1021/acs.analchem.7b04033
Wenqiang Chen 1 , Xiuxiu Yue 1 , Hui Zhang 2 , Wenxiu Li 3 , Liangliang Zhang 3 , Qi Xiao 1 , Chusheng Huang 1 , Jiarong Sheng 1 , Xiangzhi Song 2
Affiliation  

Glutathione (GSH) and hydrogen polysulfides (H2Sn) play crucial roles in many physiological processes. To unravel the complicated interrelationship and cellular cross-talk between GSH and H2Sn, the development of single-molecule fluorescent probes that can selectively sense GSH and H2Sn simultaneously from different emission channels is highly desirable. In this report, we have developed the first dual-detection fluorescent probe, ACC-SePh, which responded to GSH with green fluorescence emission, whereas it reacted with H2Sn and emitted blue fluorescence. The probe exhibited excellent selectivity and sensitivity toward GSH and H2Sn over other common reactive sulfur species, such as Cys, Hcy and H2S. Importantly, we also demonstrated that ACC-SePh can be used for dual-channel imaging of endogenous GSH and H2Sn in living RAW264.7 cells.

中文翻译:

同时检测不同排放通道中的谷胱甘肽和多硫化氢

谷胱甘肽(GSH)和多硫化氢(H 2 S n)在许多生理过程中起着至关重要的作用。为了揭示GSH和H 2 S n之间的复杂的相互关系和细胞串扰,迫切需要开发能够选择性地同时从不同的发射通道同时感测GSH和H 2 S n的单分子荧光探针。在本报告中,我们开发了首个双重检测荧光探针ACC-SePh,该探针对GSH发出绿色荧光,而与H 2 S n反应并发出蓝色荧光。该探针对GSH和H表现出出色的选择性和敏感性2小号Ñ比其它普通反应性硫物种,如半胱氨酸,同型半胱氨酸和H 2 S.重要的是,我们还证明了ACC-SEPH可用于内源性GSH和H的双通道成像2小号Ñ在活RAW264.7细胞。
更新日期:2017-11-16
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