Three Canon bench-scale bioreactors with a volume of 2 L operating in parallel were configured as submerged biofilters. In the present study we investigated the effects of a high ammonium concentration (320 mgNH₄⁺· L⁻¹) and different concentrations of organic matter (0, 100 and 400 mgCOD·L⁻¹) on the nitrogen removal capacity and the bacterial community structure. After 60 days, the Canon biofilters operated properly under concentrations of 0 and 100 mgCOD·L⁻¹ of organic matter, with nitrogen removal efficiencies up to 85%. However, a higher concentration of organic matter (400 mgCOD·L⁻¹) produced a partial inhibition of nitrogen removal (68.1% efficiency). The addition of higher concentrations of organic matter a modified the bacterial community structure in the Canon biofilter, increasing the proliferation of heterotrophic bacteria related to the genera of Thauera, Longilinea, Ornatilinea, Thermomarinilinea, unclassified Chlorobiales and Denitratisoma. However, heterotrophic bacteria co-exist with Nitrosomonas and Candidatus Scalindua. Thus, our study confirms the co-existence of different microbial activities (AOB, Anammox and denitrification) and the adaptation of a fixed-biofilm system to different concentrations of organic matter.

将三个容量为2 L的佳能台式生物反应器并联运行，配置为浸没式生物过滤器。在本研究中，我们研究了高铵浓度（320 mgNH ₄ ⁺ ·L ^-1）和不同浓度的有机物（0、100和400 mgCOD·L ^-1）对脱氮能力和细菌群落的影响结构体。60天后，佳能生物滤池在0和100 mgCOD·L ^-1的有机物浓度下正常运行，脱氮效率高达85％。但是，有机物的浓度较高（400 mgCOD·L ^-1）对脱氮产生了部分抑制作用（效率为68.1％）。加入较高浓度的有机物的的改性的细菌群落结构在佳能生物滤池，增加有关的属异养细菌的增殖Thauera，Longilinea，Ornatilinea，Thermomarinilinea，未分类Chlorobiales和Denitratisoma。但是，异养细菌与亚硝化单胞菌和斯卡林达假丝酵母共存。因此，我们的研究证实了不同微生物活动（AOB，厌氧氨氧化和反硝化作用）的共存以及固定生物膜系统对不同浓度有机物的适应性。