Enzymatic probe sonication (EPS) was investigated as a novel and alternative technology for the extraction of five Se species SeCys₂, MeSeCys, Se (IV), SeMet and Se (VI) from animal feeds. The critical parameters of EPS such as enzyme types, ultrasonic power, sample/enzyme ratio, extraction time and temperature were carefully optimized. Under current conditions, one sample can be extracted in 60s, in contrast to several hours required by the conventional enzymatic methods Moreover, the extraction is performed in pure water, no further temperature control or pH adjustment is needed, and therefore the risk of species interconvertion is drastically reduced or inhibited. The Se species were separated and quantitatively determined by high-performance liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS), which allows the separation of organic and inorganic Se species in a single chromatographic run. The newly developed method was successfully applied to the determination of Se species in 18 feed samples collected from markets and local farms. Concerning the feed samples studied, the results of this work suggest that stricter measures should be taken to control the Se-enriched feed supplements in terms of Se species.

研究了酶探针超声（EPS）作为一种新的替代技术来提取5种SeCys _2的可能性，MeSeCys，Se（IV），SeMet和Se（VI）来自动物饲料。精心优化了EPS的关键参数，如酶的类型，超声功率，样品/酶比，提取时间和温度。在当前条件下，与常规酶法所需的几个小时相比，可以在60秒内提取一个样品。此外，提取是在纯水中进行的，不需要进一步的温度控制或pH调节，因此存在物种相互转化的风险大大减少或抑制。通过高效液相色谱-氢化物发生原子荧光光谱法（HPLC-HG-AFS）分离并定量测定Se种类，从而可以在一次色谱操作中分离有机和无机Se种类。新开发的方法已成功用于测定从市场和当地农场收集的18种饲料样品中的硒种类。关于所研究的饲料样品，这项工作的结果表明应采取更严格的措施来控制富硒饲料中硒的种类。