Biomarkers are often present in complex biological fluids like blood, requiring multiple, slow sample preparation steps that pose limitations in simplifying analysis. Here we report integrated immunoaffinity extraction and separation devices for analysis of preterm birth biomarkers in a human blood serum matrix. A reactive polymer monolith was used for immobilization of antibodies for selective extraction of target preterm birth biomarkers. Microfluidic immunoaffinity extraction protocols were optimized and then integrated with microchip electrophoresis for separation. Using these integrated devices, a ∼30 min analysis was carried out on low nanomolar concentrations of two preterm birth biomarkers spiked in a human serum matrix. This work is a promising step towards the development of an automated, integrated platform for determination of preterm birth risk.

中文翻译：

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电动微流控装置，用于整合免疫亲和整体提取物和电泳分离早产生物标志物

生物标志物通常存在于复杂的生物流体（例如血液）中，需要多个缓慢的样品制备步骤，这些步骤在简化分析方面存在局限性。在这里，我们报告集成的免疫亲和提取和分离设备，用于分析人血清基质中的早产生物标志物。反应性聚合物整体被用于固定抗体，以选择性地提取目标早产生物标志物。优化了微流体免疫亲和提取方案，然后与微芯片电泳集成以进行分离。使用这些集成设备，对掺入人血清基质中的两种纳摩尔浓度低的早产生物标志物进行了约30分钟的分析。这项工作是朝着开发自动化，