In the course of optimizing pargamicin A production in Amycolatopsis sp. ML1-hF4, we discovered novel cyclic depsipeptide compounds in the broth and designated them valgamicins A, C, T and V. The structures of these molecules were determined by spectroscopic studies, advanced Marfey's method and X-ray crystal structural analysis. Valgamicin C contains the extremely rare amino acid cleonine. To our knowledge, this is the first report of a cleonine-containing metabolite from a naturally isolated microorganism without any breeding or mutation treatment. None of the valgamicins showed potent antibacterial activity against either Gram-positive or -negative bacteria. Valgamicins A, C and T exhibited moderate cytotoxicity against human tumor cell lines.The Journal of Antibiotics advance online publication, 15 November 2017; doi:10.1038/ja.2017.135.

中文翻译：

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Valgamicin C，一种新颖的环状二肽，含有不常见的氨基酸可莱因，以及由霉菌属菌种生产的相关valgamicins A，T和V。ML1-hF4。

在优化霉菌属菌种中帕加霉素A产量的过程中。ML1-hF4，我们在肉汤中发现了新的环状二肽肽化合物，并将其命名为valgamicins A，C，T和V。这些分子的结构是通过光谱研究，先进的Marfey方法和X射线晶体结构分析确定的。Valgamicin C含有极为罕见的氨基酸可莱因。据我们所知，这是未经任何繁殖或突变处理而天然分离的微生物中含有可莱因的代谢产物的首次报道。没有一种缬氨酰胺对革兰氏阳性或阴性细菌显示出有效的抗菌活性。Valgamicins A，C和T对人肿瘤细胞系表现出中度细胞毒性。《抗生素杂志》在线提前出版，2017年11月15日; doi：10.1038 / ja.2017.135。