Drug-resistant bacteria are still emerging, and screening of new skeletal antibiotics is important. During our continuous screening for antimicrobial agents, we discovered a new antimicrobial, named quadoctomycin, from solid culture of Streptomyces sp. MM168-141F8. The substance was purified by solvent extraction, silica gel chromatography and HPLC. Structural elucidation of quadoctomycin was performed by MS and NMR analyses and chemical degradation. Quadoctomycin possesses a 48-membered polyol macrolide skeleton in which an α-D-mannoside is connected to C-22 by an O-glycosidic linkage. The structure of quadoctomycin was found to be related to that of monazomycin A based on the analyses of NMR spectra in the same solvent (pyridine-d₅). Quadoctomycin showed potent antibacterial activity against Staphylococcus aureus, including methicillin-resistant S. aureus, and other Gram-positive pathogenic bacteria such as Enterococcus faecalis and E. faecium (including drug-resistant strains), but did not show activity toward Gram-negative bacteria or Candida albicans.The Journal of Antibiotics advance online publication 15 November 2017; doi:10.1038/ja.2017.140.

中文翻译：

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Quadoctomycin，一种来自Streptomyces sp。的48元大环内酯类抗生素。MM168-141F8。

耐药细菌仍在兴起，新骨骼抗生素的筛选非常重要。在我们连续筛选抗菌剂的过程中，我们从链霉菌属菌种的固体培养物中发现了一种新的抗菌素，名为Quadoctomycin。MM168-141F8。该物质通过溶剂萃取，硅胶色谱和HPLC纯化。通过MS和NMR分析以及化学降解进行了Quadoctomycin的结构鉴定。Quadoctomycin具有48元多元醇大环内酯骨架，其中α-D-甘露糖苷通过O-糖苷键与C-22连接。通过在相同溶剂（吡啶-d ₅）。Quadoctomycin对金黄色葡萄球菌（包括耐甲氧西林的金黄色葡萄球菌）和其他革兰氏阳性病原菌（如粪肠球菌和屎肠球菌（包括耐药菌株））显示出有效的抗菌活性，但对革兰氏阴性菌没有活性《抗生素杂志》于2017年11月15日提前在线出版; doi：10.1038 / ja.2017.140。