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A multiregional proteomic survey of the postnatal human brain
Nature Neuroscience ( IF 25.0 ) Pub Date : 2017-11-13 , DOI: 10.1038/s41593-017-0011-2
Becky C. Carlyle,Robert R. Kitchen,Jean E. Kanyo,Edward Z. Voss,Mihovil Pletikos,André M. M. Sousa,TuKiet T. Lam,Mark B. Gerstein,Nenad Sestan,Angus C. Nairn

Detailed observations of transcriptional, translational and post-translational events in the human brain are essential to improving our understanding of its development, function and vulnerability to disease. Here, we exploited label-free quantitative tandem mass-spectrometry to create an in-depth proteomic survey of regions of the postnatal human brain, ranging in age from early infancy to adulthood. Integration of protein data with existing matched whole-transcriptome sequencing (RNA-seq) from the BrainSpan project revealed varied patterns of protein–RNA relationships, with generally increased magnitudes of protein abundance differences between brain regions compared to RNA. Many of the differences amplified in protein data were reflective of cytoarchitectural and functional variation between brain regions. Comparing structurally similar cortical regions revealed significant differences in the abundances of receptor-associated and resident plasma membrane proteins that were not readily observed in the RNA expression data.

中文翻译:

产后人脑的多区域蛋白质组学研究

对人脑中转录,翻译和翻译后事件的详细观察对于增进我们对其发展,功能和疾病易感性的了解至关重要。在这里,我们利用无标签定量串联质谱技术对从婴儿期到成年的年龄范围内的产后人脑区域进行了深入的蛋白质组学研究。蛋白质数据与BrainSpan项目中现有的匹配全转录组测序(RNA-seq)的整合揭示了蛋白质与RNA关系的各种模式,与RNA相比,大脑区域之间蛋白质丰度差异的幅度通常会增加。蛋白质数据中放大的许多差异反映了大脑区域之间的细胞结构和功能变异。
更新日期:2017-11-13
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