A novel screen-printed cell-based electrochemical sensor was developed to assess bacterial quorum signaling molecules, N-acylhomoserine lactones (AHLs). Screen-printed carbon electrode (SPCE), which possesses excellent properties such as low-cost, disposable and energy-efficient, was modified with multi-walled carbon nanotubes (MWNTs) to improve electrochemical signals and enhance the sensitivity. Rat basophilic leukemia (RBL-2H3) mast cells encapsulated in alginate/graphene oxide (NaAgl/GO) hydrogel were immobilized on the MWNTs/SPCE to serve as recognition element. Electrochemical impedance spectroscopy (EIS) was employed to record the cell impedance signal as-influenced by Pseudomonas aeruginosa quorum-sensing molecule, N−3-oxododecanoyl homoserine lactone (3OC₁₂-HSL). Experimental results show that 3OC₁₂-HSL caused a significant decrease in cell viability in a dose dependent manner. The EIS value decreased with concentrations of 3OC₁₂-HSL in the range of 0.1–1 μM, and the detection limit for 3OC₁₂-HSL was calculated to be 0.094 μM. These results were confirmed via cell viability, SEM, TEM analysis. Next, the sensor was successfully applied to monitoring the production of AHLs by spoilage bacteria in three different freshwater fish juice samples which efficiently proved the practicability of this cell based method. Therefore, the proposed cell sensor may serve as an innovative and effective approach to the measurement of quorum signaling molecule and thus provides a new avenue for real-time monitoring the spoilage bacteria in freshwater fish production.

一种新型的丝网印刷的基于细胞的电化学传感器被开发来评估细菌群体信号分子，N-酰基高丝氨酸内酯（AHLs）。丝网印刷碳电极（SPCE）具有优异的性能，例如低成本，一次性使用和高能效，并通过多壁碳纳米管（MWNT）进行了修饰，以改善电化学信号并提高灵敏度。将封装在藻酸盐/氧化石墨烯（NaAg1 / GO）水凝胶中的大鼠嗜碱性粒细胞（RBL-2H3）肥大细胞固定在MWNTs / SPCE上，作为识别元件。采用电化学阻抗谱（EIS）记录铜绿假单胞菌群体感应分子N -3-氧十二烷酰高丝氨酸内酯（3OC ₁₂-HSL）。实验结果表明3OC ₁₂ -HSL以剂量依赖性方式引起细胞活力的显着降低。当3OC ₁₂ -HSL的浓度在0.1–1μM范围内时，EIS值降低，并且3OC ₁₂的检出限-HSL经计算为0.094μM。这些结果通过细胞活力，SEM，TEM分析得到证实。下一步，该传感器成功应用于监测三种不同淡水鱼汁样品中细菌变质引起的AHL的产生，这有效地证明了这种基于细胞的方法的实用性。因此，所提出的细胞传感器可以作为测量群体信号分子的一种创新而有效的方法，从而为实时监测淡水鱼生产中的腐败细菌提供了一条新途径。