A novel improved preconcentration method known as synergistic cloud point extraction was established for isoquercitrin preconcentration and determination in rat plasma prior to its determination by high performance liquid chromatography. Synergistic cloud point extraction greatly simplified isoquercitrin extraction and detection. This method was accomplished at room temperature (about 22 °C) in 1 min with the nonionic surfactant Tergitol TMN-6 as the extractant, n-octanol as cloud point revulsant and synergic reagent. Parameters that affect the synergistic cloud point extraction processes, such as the concentrations of Tergitol TMN-6, volume of n-octanol, sample pH, salt content and extraction time were investigated and optimized. Under the optimum conditions, the calibration curve for the analyte was linear in the range from 5 to 500 ng mL⁻¹ with the correlation coefficients greater than 0.9996. Meanwhile, limit of detection (S/N = 3) was less than 1.6 ng mL⁻¹ and limit of quantification (S/N = 10) was less than 5 ng mL⁻¹. It demonstrated that the method can be successfully applied to the pharmacokinetic investigation of isoquercitrin.

建立了一种新的改进的预浓缩方法，称为协同浊点提取，用于在高效液相色谱法测定大鼠血浆之前进行异槲皮苷的预浓缩和测定。协同浊点提取大大简化了异槲皮苷的提取和检测。该方法是在室温（约22°C）下于1分钟内用非离子表面活性剂Tergitol TMN-6作为萃取剂，正辛醇作为浊点抑制剂和协同试剂完成的。影响协同浊点提取过程的参数，例如Tergitol TMN-6的浓度，n的体积-辛醇，样品的pH，盐含量和提取时间进行了研究和优化。在最佳条件下，分析物的校准曲线在5至500 ng mL ^-1范围内呈线性，相关系数大于0.9996。同时，检测限（S / N = 3）小于1.6 ng mL ^-1，定量限（S / N = 10）小于5 ng mL ^-1。结果表明，该方法可成功应用于异槲皮苷的药代动力学研究。