The enzyme glucosamine-6-phosphate synthase (GlmS) is an important point of metabolic control in biosynthesis of amino sugar-containing macromolecules and is therefore a potential target in order to design antibacterial and antifungal drugs. It has two oligomerization states, which are the active dimer and the inactive hexamer. For the first time, the potential of CE to separate and quantify the two forms was studied. After incubating GlmS with the d-glucosamine 6-phosphate (GlcN6P) inhibitor, an electrolyte based on sodium phosphate at pH 7.2 and an ionic strength of 100 mM plus GlcN6P (either 2 or 20 mM) allowed the hexamer-dimer separation. However, the displacement of the dimer/hexamer equilibrium during the analysis time prevented any improvement of the resolution when varying the effective separation length or the temperature of the analysis. Therefore, the use of a short-end CE method allowed the decrease in the analysis time to about 1 min. Some parameters such as the temperature and the time of incubation and the ratio of the inhibitor and enzyme concentrations were studied. Then, it was also possible to test, very rapidly and with a very small amount, some molecules having an inhibition potential for the GlmS enzyme (arabinose-5-phosphate oxime, 2-amino-2-deoxy-d-glucitol 6-phosphate, and glucose-6-phosphate).

氨基葡萄糖6磷酸合酶（GlmS）是生物合成含氨基糖的大分子中代谢控制的重要方面，因此是设计抗菌和抗真菌药物的潜在目标。它具有两个低聚状态，分别是活性二聚体和非活性六聚体。首次研究了CE分离和量化这两种形式的潜力。用d孵育GlmS之后-葡糖胺6-磷酸酯（GlcN6P）抑制剂，基于磷酸钠的pH 7.2电解质和100 mM的离子强度加上GlcN6P（2或20 mM）允许六聚体-二聚体分离。但是，在分析时间内二聚体/六聚体平衡的位移阻止了在改变有效分离长度或分析温度时分辨率的任何改善。因此，使用短时CE方法可以将分析时间减少到大约1分钟。研究了一些参数，例如温度和孵育时间以及抑制剂与酶浓度的比值。然后，还可以非常快速且少量地测试一些对GlmS酶具有抑制潜能的分子（阿拉伯糖5磷酸肟，2-氨基2-脱氧-d-葡萄糖醇6-磷酸酯和葡萄糖-6-磷酸酯）。