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Epithelial organoid cultures from patients with ulcerative colitis and Crohn's disease: a truly long-term model to study the molecular basis for inflammatory bowel disease?
Gut ( IF 24.5 ) Pub Date : 2017-02-03 , DOI: 10.1136/gutjnl-2016-313667
Manuel Noben , Bram Verstockt , Magali de Bruyn , Nikolai Hendriks , Gert Van Assche , Séverine Vermeire , Catherine Verfaillie , Marc Ferrante

With great interest we read the paper by Dotti et al ,1 who identified a set of differentially expressed genes in epithelial organoid cultures (EpOCs) derived from patients with UC compared with EpOCs from healthy controls. Similarly, we created an EpOC library from patients with UC (n=17), Crohn's disease (CD, n=12) and healthy controls (n=10). First, we assessed the potential of isolated intestinal crypts to grow into organoids (colony forming units). We observed that a similar percentage of organoids was formed from intestinal crypts isolated from controls and patients with UC or CD. Moreover, intestinal crypts isolated from macroscopically inflamed and non-inflamed tissue had similar potential to form organoids (figure 1A). Although we used a slightly divergent differentiation medium,2 the expansion and differentiation capacity of our organoids was similar as demonstrated by Dotti et al . Figure 1 (A) Colony forming units (CFUs) determined by quantification of the number of seeded crypts at the day of isolation and organoid formation after 7 days. CFUs are expressed as a percentage of organoids representative to the original number of seeded crypts. (B) Relative mRNA expression levels of leucine-rich repeat-containing G-protein coupled receptor 5 ( LGR5 ), mucin2 ( MUC2 ) and protein atonal homologue 1 ( ATOH1 ). mRNA levels were …

中文翻译:

溃疡性结肠炎和克罗恩病患者的上皮类器官培养:研究炎症性肠病分子基础的真正长期模型?

我们怀着极大的兴趣阅读了 Dotti 等人 1 的论文,他在来自 UC 患者的上皮类器官培养物 (EpOCs) 与健康对照的 EpOCs 相比确定了一组差异表达的基因。同样,我们从 UC(n=17)、克罗恩病(CD,n=12)和健康对照(n=10)患者创建了一个 EpOC 库。首先,我们评估了孤立的肠隐窝生长成类器官(集落形成单位)的潜力。我们观察到,从对照和 UC 或 CD 患者分离的肠隐窝形成了相似百分比的类器官。此外,从宏观发炎和非发炎组织中分离出的肠隐窝具有相似的形成类器官的潜力(图 1A)。虽然我们使用了稍微不同的分化培养基,2 我们的类器官的扩展和分化能力与 Dotti 等人所证明的相似。图 1 (A) 菌落形成单位 (CFU) 通过量化隔离当天的种子隐窝数量和 7 天后的类器官形成。CFU 表示为有机体占种子隐窝原始数量的百分比。(B) 富含亮氨酸重复序列的 G 蛋白偶联受体 5 (LGR5)、mucin2 (MUC2) 和蛋白无调同源物 1 (ATOH1) 的相对 mRNA 表达水平。mRNA 水平是…… CFU 表示为有机体占种子隐窝原始数量的百分比。(B) 富含亮氨酸重复序列的 G 蛋白偶联受体 5 (LGR5)、mucin2 (MUC2) 和蛋白无调同源物 1 (ATOH1) 的相对 mRNA 表达水平。mRNA 水平是…… CFU 表示为有机体占种子隐窝原始数量的百分比。(B) 富含亮氨酸重复序列的 G 蛋白偶联受体 5 (LGR5)、mucin2 (MUC2) 和蛋白无调同源物 1 (ATOH1) 的相对 mRNA 表达水平。mRNA 水平是……
更新日期:2017-02-03
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