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Detection of early stage prostate cancer by using a simple carbon [email protected] biosensor
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2017-11-10 , DOI: 10.1016/j.bios.2017.11.035
Sungkyung Ji , Myeongsoon Lee , Don Kim

This study is an investigation for an inexpensive, simple and sensitive biosensor to detect prostate cancer using bioactivated-multi wall carbon nanotubes (MWCNTs, diameter of 20 nm, length of 5 µm) and a micro-pore filter paper (pore size of 0.45 µm). For the immunoassay of prostate specific antigen (PSA), which is a biomarker of prostate cancer, MWCNTs were activated with PSA antibody (monoclonal antibody of the prostate specific antigen) by using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt (NHSS). The activated MWCNTs were deposited on the micro-pore filter paper to use as a biosensor. The prepared biosensor can assay from 0 to 500 ng/mL of PSA level within 2 h with the detection limit of 1.18 ng/mL by the measurement of resistance change. The resistance change was caused by site selective interaction between PSA and PSA-antigen with an inexpensive bench top digital multimeter (5 1/2 digits). The detection range and sensitivity of the prepared sensor are good enough to diagnose the early stage of prostate cancer (> 4 ng/mL of PSA). This paper-based biosensor is about 20 times cheaper (fabricated biosensor price: 2.4 $) and over 10 times faster than enzyme-linked immunosorbent assay (ELISA), which is a general method for the detection of a specific protein in the modernized hospitals. Furthermore, the maximum detection limit is about 50 times higher than ELISA.



中文翻译:

使用简单的碳[受电子邮件保护的]生物传感器检测早期前列腺癌

这项研究是对廉价,简单且灵敏的生物传感器进行的研究,该传感器使用生物活化的多壁碳纳米管(MWCNT,直径为20 nm,长度为5 µm)和微孔滤纸(孔径为0.45 µm)来检测前列腺癌)。对于作为前列腺癌生物标志物的前列腺特异性抗原(PSA)的免疫测定,通过使用N-(3-二甲基氨基丙基)-N'-乙基碳二亚胺盐酸盐( EDC)和N-羟基磺基琥珀酰亚胺钠盐(NHSS)。活化的MWCNT沉积在微孔滤纸上,用作生物传感器。制备的生物传感器可在2 h内通过测量电阻变化,检测PSA含量为0至500 ng / mL,检测极限为1.18 ng / mL。电阻变化是由PSA和PSA抗原之间的位点选择性相互作用(使用便宜的台式数字万用表(5 1/2位))引起的。所准备传感器的检测范围和灵敏度足以诊断前列腺癌的早期阶段(> 4 ng / mL PSA)。这种基于纸张的生物传感器便宜约20倍(预制生物传感器价格:2.4美元),比酶联免疫吸附测定(ELISA)快10倍以上,后者是现代化医院中检测特定蛋白质的通用方法。此外,最大检测限比ELISA高约50倍。所制备传感器的检测范围和灵敏度足以诊断前列腺癌的早期阶段(> 4 ng / mL PSA)。这种基于纸张的生物传感器便宜约20倍(预制生物传感器价格:2.4美元),比酶联免疫吸附测定(ELISA)快10倍以上,后者是现代化医院中检测特定蛋白质的通用方法。此外,最大检测限比ELISA高约50倍。所准备传感器的检测范围和灵敏度足以诊断前列腺癌的早期阶段(> 4 ng / mL PSA)。这种基于纸张的生物传感器便宜约20倍(预制生物传感器价格:2.4美元),比酶联免疫吸附测定(ELISA)快10倍以上,后者是现代化医院中检测特定蛋白质的通用方法。此外,最大检测限比ELISA高约50倍。

更新日期:2017-11-10
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