The Anelloviridae comprises single-stranded DNA viruses currently grouped in sixty-eight species classified in twelve genera. They have been found in many vertebrate hosts including primates. In this study, we describe the application of the high-throughput sequencing to examine the frequency and diversity of anelloviruses in rodents, bats and opossums captured in São Paulo State, Brazil. We report a total of twenty-six anelloviruses with sixteen nearly complete genomes and ten partial genomes, which include eleven potential novel species identified in rodents (Cricetidae), bats (Molossidae and Phyllostomidae), and opossums (Didelphidae). We also propose the inclusion of two potential new genera within the Anelloviridae family, provisionally named Omegatorquevirus and Sigmatorquevirus, including six and three novel species of anelloviruses, respectively. In summary, this study expands the diversity and the host range of the known anelloviruses.

Anelloviridae包含单链DNA病毒，目前分为12个属的68个物种。它们已在包括灵长类在内的许多脊椎动物寄主中被发现。在这项研究中，我们描述了高通量测序的应用，以检查在巴西圣保罗州捕获的啮齿动物，蝙蝠和负鼠中的网膜病毒的频率和多样性。我们报告了总共26种网纹病毒，它们具有16个近乎完整的基因组和10个部分基因组，其中包括在啮齿动物（Cricetidae），蝙蝠（Molossidae和Phyllostomidae）和负鼠（Didelphidae）中鉴定出的11种潜在的新物种。我们还建议将Anelloviridae家族中两个潜在的新属包括在内，临时命名为Ω巨噬病毒和Sigmatorquevirus，分别包括6种和3种新的网膜病毒。总而言之，这项研究扩展了已知的网膜病毒的多样性和宿主范围。