Several studies suggest that potential competition exists between marine cations and heavy metals for binding sites on the cell wall of mosses. This competition would impact the heavy metal concentration measured in mosses by biomonitoring programs, which may underestimate air pollution by heavy metals in a coastal environment. In the present study, we aim to identify possible mechanisms affecting lead uptake by mosses in a coastal environment, specifically, the competition between lead (Pb²⁺) and sodium (Na⁺) for binding sites in Hypnum cupressiforme (Hc). We also compared the response of continental and coastal Hc populations to Pb²⁺ exposure by immersing the moss samples in artificial solutions that comprised six experimental treatments and subsequently locating and quantifying Pb²⁺ and Na⁺ using the sequential elution technique and X-ray microanalyses with a scanning electron microscope. We demonstrated that high concentrations of Pb²⁺ prevented Na⁺ from binding to the cell wall. We also examined the effect of the salt acclimation of Hc on Pb²⁺ and Na⁺ accumulation. Coastal Hc populations accumulated more Na and less Pb than continental Hc populations in all treatments. Moreover, our results showed treatment effects on the intra/extracellular distribution of Na⁺, as well as site. This feedback on the influence of salt stress tolerance on Pb²⁺ uptake by mosses requires further study and can be investigated for other heavy metals, leading to a better use of mosses as biomonitoring tools.

多项研究表明，海洋阳离子与重金属之间存在潜在竞争，竞争的是苔藓细胞壁上的结合位点。这种竞争将影响通过生物监测计划在苔藓中测得的重金属浓度，这可能会低估沿海环境中重金属对空气的污染。在本研究中，我们旨在确定可能的机制，以影响沿海环境中的苔藓对铅的吸收，特别是铅（Pb ²⁺）和钠（Na ⁺）之间的竞争，从而影响了杯形茄形（Hc）中的结合位点。我们还比较了大陆和沿海Hc种群对Pb ²⁺的响应通过将苔藓样品浸入包含六种实验处理方法的人工溶液中进行暴露，然后使用顺序洗脱技术和扫描电子显微镜对X射线显微分析，对Pb ²⁺和Na ⁺进行定位和定量。我们证明了高浓度的Pb ²⁺阻止了Na ⁺结合到细胞壁上。我们还检查了Hc的盐分驯化对Pb ²⁺和Na ⁺积累的影响。在所有处理中，沿海HC人群积累的Na和Pb少于大陆HC人群。此外，我们的结果表明治疗对Na ^+的胞内/胞外分布有影响，以及网站。关于盐胁迫耐受性对苔藓对Pb ²⁺吸收的影响的反馈需要进一步研究，并且可以对其他重金属进行研究，从而导致更好地使用苔藓作为生物监测工具。