Propamocarb (PM) is a widely used fungicide with property of affecting fatty acid and phospholipid biosynthesis in funguses. In this study, we explored its effects on mice gut microbiota and metabolism by exposing mice to 3, 30, and 300 mg/L PM through drinking water for a duration of 28 days. We observed that the transcription of hepatic genes related to regulate lipid metabolism were perturbed by PM exposure. The microbiota in the cecal contents and feces changed during or after PM exposure at phylum or genus levels. 16S rRNA gene sequencing for the cecal content revealed shifted in overall microbial structure after PM exposure, and operational taxonomic unit (OTU) analysis indicated that 32.2% of OTUs changed by 300 mg/mL PM exposure for 28 days. In addition, based on ¹H NMR analysis,a total of 20 fecal metabolites mainly including succinate, short chain fatty acids, bile acids and trimethylamine were found to be significantly influenced by exposure to 300 mg/L PM.,. These metabolites were tightly correlated to host metabolism. Our findings indicated that high doses of PM exposure could disturb mice metabolism through, or partly through, altering the gut microbiota and microbial metabolites.

丙碳威（PM）是一种广泛使用的杀菌剂，具有影响真菌中脂肪酸和磷脂生物合成的特性。在这项研究中，我们通过将小鼠暴露于3、30和300 mg / L PM的饮用水中28天，探讨了其对小鼠肠道菌群和代谢的影响。我们观察到，与PM接触会扰乱与调节脂质代谢相关的肝基因的转录。盲肠内容物和粪便中的微生物群在门或属水平的PM暴露期间或之后发生变化。盲肠含量的16S rRNA基因测序揭示了PM暴露后整体微生物结构发生了变化，而操作分类单位（OTU）分析表明，在28天的300 mg / mL PM暴露下，有32.2％的OTU发生了变化。另外，根据¹1 H NMR分析表明，总共300种粪便代谢物主要受琥珀酸，短链脂肪酸，胆汁酸和三甲胺的影响，其暴露于300 mg / L PM。会受到显着影响。这些代谢产物与宿主代谢密切相关。我们的研究结果表明，高剂量的PM暴露可能会通过或部分通过改变肠道菌群和微生物代谢产物来干扰小鼠的新陈代谢。