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Chip-based magnetic solid phase microextraction coupled with ICP-MS for the determination of Cd and Se in HepG2 cells incubated with CdSe quantum dots
Talanta ( IF 6.1 ) Pub Date : 2017-11-10 , DOI: 10.1016/j.talanta.2017.11.013
Xiaoxiao Yu , Beibei Chen , Man He , Han Wang , Bin Hu

The quantification of trace Cd and Se in cells incubated with CdSe quantum dots (QDs) is critical to investigate the cytotoxicity of CdSe QDs. In this work, a miniaturized platform, namely chip-based magnetic solid phase microextraction (MSPME) packing with sulfhydryl group functionalized magnetic nanoparticles, was fabricated and combined with inductively coupled plasma mass spectrometry (ICP-MS) for the determination of trace Cd and Se in cells. Under the optimized conditions, the limits of detection (LOD) of the developed chip-based MSPME-ICP-MS system are 2.2 and 21 ng L−1 for Cd and Se, respectively. The proposed method is applied successfully to the analysis of total and released small molecular fraction of Cd and Se in Human hepatocellular carcinoma cells (HepG2 cells) incubated with CdSe QDs, and the recoveries for the spiked samples are in the range of 86.0–109%. This method shows great promise to analyze cell samples and the obtained results are instructive to explore the cytotoxicity mechanism of CdSe QDs in cells.



中文翻译:

基于芯片的磁性固相微萃取结合ICP-MS测定与CdSe量子点孵育的HepG2细胞中的Cd和Se

用CdSe量子点(QDs)孵育的细胞中痕量Cd和Se的定量对于研究CdSe QD的细胞毒性至关重要。在这项工作中,制造了一个小型平台,即带有巯基基团官能化的磁性纳米粒子的基于芯片的磁性固相微萃取(MSPME)填料,并与电感耦合等离子体质谱法(ICP-MS)结合用于测定痕量Cd和Se在细胞中。在优化的条件下,已开发的基于芯片的MSPME-ICP-MS系统的检测限(LOD)为2.2和21 ng L -1分别用于镉和硒。所提出的方法已成功地用于分析与CdSe QDs孵育的人肝癌细胞(HepG2细胞)中Cd和Se的总和释放的小分子部分,加标样品的回收率在86.0–109%的范围内。该方法对细胞样品的分析具有广阔的前景,所获得的结果对探索CdSe量子点在细胞中的细胞毒性机制具有指导意义。

更新日期:2017-11-10
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