Alginate and polycaprolactone (PCL) were coelectrospun using a dual-jet system to prepare composite nanofibers in defined ratios, and hence both chemical properties and hydrophobicity of scaffolds can be manipulated. These nanofibers were applied in gene immobilization: positive charged polyethyleneimine (PEI)/DNA polyplexes were adsorbed onto anionic alginate fibers, and the higher ratios of alginate resulted in the more immobilized nonviral vectors. Through the incorporation of PCL, biocompatibility of scaffolds was highly improved. Finally, these scaffolds were used for in situ transfection application. Compared to pure alginate fibers, composite fibers not only successfully transferred target genes to adhered cells but also enhanced cell morphology and viability, suggesting that alginate/PCL nanofibers were multifunctional with gene delivery capability and biocompatibility, and the manipulation of their composition can balance and optimize both requirements. To our knowledge, this approach might be the first one using electrostatic interactions to immobilize genes onto nanofibrous scaffolds for in situ transfection application.

使用双射流系统将藻酸盐和聚己内酯（PCL）共电纺丝，以定义的比例制备复合纳米纤维，因此可以控制支架的化学性质和疏水性。这些纳米纤维被用于基因固定化：带正电的聚乙烯亚胺（PEI）/ DNA复合物被吸附在阴离子藻酸盐纤维上，藻酸盐的比例越高，固定化的非病毒载体越多。通过掺入PCL，极大地提高了支架的生物相容性。最后，这些脚手架用于原位转染应用程序。与纯藻酸盐纤维相比，复合纤维不仅可以成功地将目标基因转移到粘附细胞上，而且还可以增强细胞的形态和生存能力，这表明藻酸盐/ PCL纳米纤维具有多功能性，具有基因传递能力和生物相容性，并且对其组成的操纵可以平衡和优化。这两个要求。据我们所知，这种方法可能是第一个使用静电相互作用将基因固定在纳米纤维支架上进行原位转染的方法。