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Structural characterization and osteogenic bioactivity of a sulfated polysaccharide from pacific abalone (Haliotis discus hannai Ino)
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2017-11-06 , DOI: 10.1016/j.carbpol.2017.11.022
Shuang Song , Bao Zhang , Sufeng Wu , Lu Huang , Chunqing Ai , Jinfeng Pan , Yi-Cheng Su , Zhongfu Wang , Chengrong Wen

Bone morphogenic protein-2 (BMP-2) is known to promote osteogenesis. To find novel adjuvants to enhance the activity of BMP-2, the present study investigated the structure BMP-2-induced osteogenic activity of a water-soluble polysaccharide from the gonad of pacific abalone (Haliotis discus hannai Ino) named AGSP. Through analysis of aldobiouronic acids released from AGSP, and monosaccharide composition comparison of AGSP and its reduced product, and methylation analysis and NMR analysis of AGSP and its desulfated derivative, the main structure residue of AGSP was determined as →3)-GlcA(1 → 3)-Gal(1→ with sulfated branches comprised of prevelant Gal and minor Glc, and →4)-β-GlcA(1 → 2)-α-Man(1→ residue were also found. AGSP possessed a sulfate content of 12.4% with a relative molecular weight of 6.6 kDa. AGSP strengthened alkaline phosphatase activity induced by BMP-2 in a dose dependent manner at 10–200 μg/mL with 425% enhancement being observed at 200 μg/mL, indicating AGSP could be an adjuvant candidate to enhance osteogenic activity of BMP-2.



中文翻译:

结构表征和从太平洋鲍鱼一个硫酸多糖的成骨活性(皱纹盘鲍鲍)

已知骨形态发生蛋白2(BMP-2)可促进成骨。为了找到新的佐剂来增强BMP-2的活性,本研究调查了结构BMP-2诱导的来自太平洋鲍鱼性腺(Haliotis discus hannai)的水溶性多糖的成骨活性。Ino)命名为AGSP。通过分析从AGSP释放的醛糖二酸,AGSP及其还原产物的单糖组成比较,AGSP及其脱硫衍生物的甲基化分析和NMR分析,确定AGSP的主要结构残基为→3)-GlcA(1→ 3)-Gal(1→带有硫酸酯化的分支,由旺盛的Gal和次要Glc组成,以及→4)-β-GlcA(1→2)-α-Man(1→残基),AGSP的硫酸盐含量为12.4。 %,相对分子质量为6.6 kDa。AGSP增强了BMP-2诱导的碱性磷酸酶活性,剂量依赖性在10–200μg/ mL时增强,在200μg/ mL时观察到425%增强,表明AGSP可能是佐剂增强BMP-2成骨活性的候选药物。

更新日期:2017-11-10
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