Soybean mosaic virus (SMV), a member of the genus Potyvirus, significantly reduces soybean production worldwide. Rsv3, which confers strain-specific resistance to SMV, was previously mapped between the markers A519F/R and M3Satt in chromosome 14 of the soybean [Glycine max (L.) Merr.] genotype L29. Analysis of the soybean genome database revealed that five different NBS-LRR sequences exist between the flanking markers. Among these candidate Rsv3 genes, the full-length cDNA of the Glyma.14g204700 was successfully cloned from L29. Over-expression of Glyma.14g204700 in leaves inoculated with SMV inhibited viral infection in a soybean genotype lacking Rsv3. In addition, the transient silencing of the candidate gene caused a high accumulation of an avirulent strain in L29 carrying Rsv3. Our results therefore provide additional line of evidence to support that Glyma.14g204700 is likely Rsv3 gene that confers strain-specific resistance to SMV.

大豆花叶病毒（SMV）是波多病毒（Potyvirus）的成员，大大降低了全世界的大豆产量。RSV3，其赋予菌株特异性到SMV电阻，先前映射之间的标记物在大豆的14染色体A519F / R和M3Satt [大豆（L.）MERR。]基因型L29。大豆基因组数据库的分析表明，在侧翼标记之间存在五个不同的NBS-LRR序列。在这些候选Rsv3基因中，成功地从L29克隆了Glyma.14g204700的全长cDNA 。在缺乏Rsv3的大豆基因型中，接种SMV的叶片中Glyma.14g204700的过表达抑制了病毒感染。另外，候选基因的瞬时沉默导致携带Rsv3的L29中无毒力菌株的高积累。因此，我们的结果提供了更多的证据来支持Glyma.14g204700很可能是Rsv3基因，赋予了菌株对SMV的特异性抗性。