Chicken anemia virus (CAV) is a small, single-stranded DNA virus of Anelloviridae family. Its genome segments encode three proteins, VP1, VP2, and VP3. This study identified an interaction between VP2 and VP3 and mapped the interaction domains. Through the yeast two-hybrid (Y2H) system, VP2 was found to interact with VP3. The presence of the VP2–VP3 complex in CAV-infected chicken cells was confirmed by co-immunoprecipitation. Confocal microscopy showed that VP2 and VP3 were expressed in the cytoplasm in cotransfected Vero cells. In the Y2H system, the interaction domains were identified as being within the N-terminal aa 1–30 and C-terminal aa 17–60 for VP2 and the N-terminal aa 46–60 and C-terminal aa 1–7 for VP3. This study showed the interaction between VP2 and VP3 of CAV and identified multiple independent interactive domains within the two proteins. This provides novel information for investigating the biological functions of these proteins.

鸡贫血病毒（CAV）是一种小型单链无疟原虫DNA病毒家庭。它的基因组片段编码三种蛋白质，VP1，VP2和VP3。这项研究确定了VP2和VP3之间的交互作用，并映射了交互作用域。通过酵母双杂交（Y2H）系统，发现VP2与VP3相互作用。通过共免疫沉淀法证实了CAV感染的鸡细胞中VP2-VP3复合物的存在。共聚焦显微镜显示，VP2和VP3在共转染的Vero细胞的细胞质中表达。在Y2H系统中，相互作用域被确定为在VP2的N末端aa 1–30和C末端aa 17–60内，而在VP3的N末端aa 46–60和C末端aa 1–7内。这项研究显示了CAV的VP2和VP3之间的相互作用，并鉴定了这两种蛋白中的多个独立的相互作用域。