The present work aims at developing two LC-HRMS setups for the screening of organic matter in astrophysical samples. Their analytical development has been demonstrated on a 100-µg residue coming from the photo-thermo chemical processing of a cometary ice analog produced in laboratory.

The first 1D-LC-HRMS setup combines a serially coupled columns configuration with HRMS detection. It has allowed to discriminate among different chemical families (amino acids, sugars, nucleobases and oligopeptides) in only one chromatographic run without neither a priori acid hydrolysis nor chemical derivatisation. The second setup is a dual-LC configuration which connects a series of trapping columns with analytical reverse-phase columns. By coupling on-line these two distinct LC units with a HRMS detection, high mass compounds (350 < m/z < 600) have been efficiently preconcentrated, separated and detected.

Our strategies demonstrate a real interest for the analysis of astrophysical samples coming in minute quantities and allowing only few analytical runs. Besides its relevance for astrobiological studies, this work points out the suitability of these two novel LC-HRMS strategies for untargeted analysis of complex environmental samples.

The present work aims at developing two LC-HRMS setups for the screening of organic matter in astrophysical samples. Their analytical development has been demonstrated on a 100-µg residue coming from the photo-thermo chemical processing of a cometary ice analog produced in laboratory.

第一个1D-LC-HRMS设置将串行耦合的列配置与HRMS检测结合在一起。无需先验酸水解或化学衍生化，只需一次色谱操作即可区分不同的化学家族（氨基酸，糖，核碱基和寡肽）。第二种设置是双LC配置，它将一系列捕集柱与分析型反相柱连接起来。通过在线连接这两个不同的LC单元和HRMS检测器，可以有效地对富集的化合物（350 < m / z <600）进行有效的预浓缩，分离和检测。

我们的策略对分析微量天体样品并只允许进行很少的分析运行表现出了浓厚的兴趣。除了与天体生物学研究相关之外，这项工作还指出了这两种新颖的LC-HRMS策略对复杂环境样品的非目标分析的适用性。