Phospholipids and sphingomyelin have a central role in infant nutrition, phospholipid acting as a nutrient carrier of long chain polyunsaturated fatty acids and sphingomyelin having an important role in cognitive function. However, analytical methods to precisely characterize and quantify these compounds in maternal milk are needed. Phospholipids and sphingomyelin were extracted using chloroform and methanol and separated on Polaris 3 Si column 250 × 2.0 mm from Varian and analyzed by high performance liquid chromatography (HPLC) coupled with mass spectrometer detector (MS). The analytical method was validated and repeatability, intermediate reproducibility, and recovery values were calculated. The relative standard deviation of repeatability (CV(r)) and intermediate reproducibility (CV(iR)) values ranged between 2.3 and 7.2% and 9.5 and 17.8%, respectively and the recovery values between 96 and 109%. Finally, the validated method was tested on human milk samples and on infant formula which were analysed also by HPLC coupled with evaporative light scattering detector (ELSD). In human milk, sphingomyelin (9.28 mg 100 mL⁻¹) was the most abundant compound, followed by phosphatidylcholine (5.39 mg 100 mL⁻¹), phosphatidylethanolamine (2.85 mg 100 mL⁻¹) and phosphatidylinositol (1.82 mg 100 mL⁻¹).

磷脂和鞘磷脂在婴儿营养中起着中心作用，磷脂充当长链多不饱和脂肪酸的营养载体，鞘磷脂在认知功能中起重要作用。但是，需要精确分析和定量母乳中这些化合物的分析方法。使用氯仿和甲醇萃取磷脂和鞘磷脂，并在250×2.0 mm的Polaris 3 Si柱上从Varian分离，并通过高效液相色谱（HPLC）和质谱检测器（MS）进行分析。验证了该分析方法，并计算了重复性，中间再现性和回收率值。重复性（CV（r））和中间再现性（CV（iR））值的相对标准偏差范围为2.3至7.2％和9.5至17.8％，回收率分别在96％和109％之间。最后，对人乳样品和婴儿配方奶粉进行了验证的方法测试，还通过HPLC和蒸发光散射检测器（ELSD）对其进行了分析。在人乳中，鞘磷脂（9.28 mg 100 mL^-1）是最丰富的化合物，其次是磷脂酰胆碱（5.39 mg 100 mL ^-1），磷脂酰乙醇胺（2.85 mg 100 mL ^-1）和磷脂酰肌醇（1.82 mg 100 mL ^-1）。