The steady increase of diabetes is becoming a major burden on health care systems. As diabetic complications arise from oxidative stress, an antioxidant therapy along with anti-diabetic drugs is recommended. Myrmecodia or ant plant is highly valued as a traditional medicine in West Papua. It is used as an alternative treatment for diabetes, as the substances produced by ants can reduce blood sugar levels. The aim of this study was to develop and establish high-performance thin-layer chromatographic (HPTLC)-bioautographic methods to measure the antioxidant and hypoglycemic effects in different extracts from Myrmecodia platytyrea and to compare them with sterol content. Antioxidant activity in methanol, ethanol, dichloromethane (DCM) and ethyl acetate (EA) extracts were measured with a direct HPTLC-2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay, while hypoglycemic effects were assessed using a newly developed α-amylase inhibitory activity assay. Stigmasterol is observed, after derivatization with anisaldehyde, as purple colored zones under visible light at hRF values of 0.66. The highest antioxidant activity was observed in the ethanol extract which is rich in polyphenols and flavonoids, while the DCM extract did not show antioxidant activity, but had significant α-amylase inhibitory activity. The highest α-amylase inhibitory activity was observed in the EA and DCM extracts and was related to their stigmasterol content.

糖尿病的持续增加正成为医疗保健系统的主要负担。由于糖尿病并发症是由氧化应激引起的，因此建议使用抗氧化剂和抗糖尿病药物。Myrmecodia或蚂蚁植物在西巴布亚被高度评价为传统药物。它被用作糖尿病的替代疗法，因为蚂蚁产生的物质可以降低血糖水平。这项研究的目的是开发和建立高性能薄层色谱（HPTLC）-生物自显影方法，以测量桔梗Myrmecodia platytyrea不同提取物中的抗氧化和降血糖作用并将它们与固醇含量进行比较。通过直接HPTLC-2,2-二苯基-1-吡啶并肼基自由基（DPPH ）测定法测定了甲醇，乙醇，二氯甲烷（DCM）和乙酸乙酯（EA）提取物中的抗氧化活性，同时使用新开发的方法评估了降血糖作用α-淀粉酶抑制活性测定。在用茴香醛衍生化之后，在hRF值为0.66的可见光下观察到紫丁香醇为紫色区域。在富含多酚和类黄酮的乙醇提取物中观察到最高的抗氧化活性，而DCM提取物没有显示抗氧化活性，但具有明显的α-淀粉酶抑制活性。在EA和DCM提取物中观察到最高的α-淀粉酶抑制活性，并且与它们的豆甾醇含量有关。