Typically, a target compound is selected as a template for a molecularly imprinted polymer (MIP); however, some target compounds are not suitable as templates because of their poor solubility. Using the tailoring properties of a deep eutectic solvent (DES), the insoluble target compound caffeic acid was transformed into a ternary choline chloride–caffeic acid–ethylene glycol (ChCl–CA–EG) DES, which was then employed as a template to prepare MIPs. The ternary DES-based MIPs were characterized by Fourier transform infrared spectroscopy, elemental analysis, scanning electron microscopy, and atomic force microscopy. The effects of time, temperature, ionic strength, and pH on the recognition processes for four polyphenols (caffeic acid, protocatechuic acid, catechin, and epicatechin) by 13 ChCl–CA–EG ternary DES-based MIPs was investigated using high-performance liquid chromatography. The recognition specificity of the MIPs for CA was significantly better than that for the other polyphenols, and the MIPs exhibited obvious characteristics of chromatographic packing materials. In addition, the recognition processes mainly followed a second-order kinetics model and the Freundlich isotherm model, which together indicated that the MIPs mainly recognized the polyphenols by chemical interactions including ion exchange, electron exchange, and new bond formation. Furthermore, the specific recognition ability of the MIPs for polyphenols, which was better than those of C₁₈, C₈, or non-molecularly imprinted polymer adsorbents, was successfully applied to the recognition of polyphenols in a Radix asteris sample. The transformation of an insoluble target compound in a polymeric DES for MIP preparation and recognition is a novel and feasible strategy suitable for use in further MIP research developments.

通常，选择目标化合物作为分子印迹聚合物（MIP）的模板；但是，某些目标化合物由于其较差的溶解性而不适合用作模板。利用深共熔溶剂（DES）的定制特性，将不溶的目标化合物咖啡酸转化为三元氯化胆碱-咖啡酸-乙二醇（ChCl-CA-EG）DES，然后将其用作模板制备MIP。通过傅立叶变换红外光谱，元素分析，扫描电子显微镜和原子力显微镜对三元DES基MIP进行了表征。时间，温度，离子强度和pH值对四种多酚（咖啡酸，原儿茶酸，儿茶素，和表儿茶素）由13个ChCl–CA–EG三元DES基MIP使用高效液相色谱法进行了研究。MIPs对CA的识别特异性明显优于其他多酚，并且MIPs具有色谱填料的明显特征。此外，识别过程主要遵循二阶动力学模型和Freundlich等温模型，两者共同表明MIP主要通过化学相互作用（包括离子交换，电子交换和新的键形成）识别多酚。此外，MIP对多酚的特异性识别能力优于C MIPs具有色谱填料的明显特征。此外，识别过程主要遵循二阶动力学模型和Freundlich等温模型，两者共同表明MIP主要通过化学相互作用（包括离子交换，电子交换和新的键形成）识别多酚。此外，MIP对多酚的特异性识别能力优于C MIPs具有色谱填料的明显特征。此外，识别过程主要遵循二阶动力学模型和Freundlich等温模型，两者共同表明MIP主要通过化学相互作用（包括离子交换，电子交换和新的键形成）识别多酚。此外，MIP对多酚的特异性识别能力优于C₁₈，C ₈或非分子印迹聚合物吸附剂已成功应用于识别紫Rad样品中的多酚。用于MIP制备和识别的聚合物DES中不溶性目标化合物的转化是一种适用于进一步MIP研究开发的新颖可行的策略。