As increasing numbers of cell-based therapies advance through clinical trials towards approval for use in humans, the need for robust and well-characterized cell culturing strategies is becoming increasingly apparent. We discuss here novel stem cell bioprocessing strategies based on the concept of using Waddington’s epigenetic landscape to represent the process of cellular decision-making during development. Specifically, we focus on the manner in which cell behavior triggers cell signaling pathways related to the initial cell fate decision. We also consider how various biochemical engineering strategies can be used to develop these bioprocesses to further enhance their utility in research and therapy.

随着越来越多的基于细胞的疗法通过临床试验逐步走向批准用于人类，对健壮且特征明确的细胞培养策略的需求变得越来越明显。我们在这里讨论基于使用Waddington的表观遗传学景观来代表开发过程中细胞决策过程的概念的新颖干细胞生物加工策略。具体而言，我们专注于细胞行为触发与初始细胞命运决定有关的细胞信号传导途径的方式。我们还考虑了如何使用各种生化工程策略来开发这些生物过程，以进一步增强其在研究和治疗中的效用。