In this paper, we develop a low-cost size-based microfluidic chip using conventional polycarbonate membrane to isolate CTCs from blood, and propose a strategy to increase the capture efficiency before cell filtration by a size enlargement method utilizing modified microbeads specifically binding to CTCs. Up to 91% of target cells were isolated from whole blood samples using our microfluidic capture system at a flow rate of 1 mL/min. Moreover, a WBC depletion process is introduced which greatly decreases the WBC retaining on the filter membrane. The tests of immunofluorescence analysis of cells captured on the membrane were performed, which demonstrates that the device could provide a dependable CTC identification and CTC count in whole blood samples. Finally, the device was further validated in the detection of CTCs from blood samples of cancer patients, and it indicates a promising capability to detect CTC response to treatment.

在本文中，我们开发了一种低成本的基于尺寸的微流控芯片，该芯片使用常规的聚碳酸酯膜从血液中分离出四氯化碳，并提出了一种策略，可通过使用与微四氯化碳特异性结合的修饰微珠进行尺寸扩大的方法来提高细胞过滤前的捕获效率。使用我们的微流体捕获系统，以1 mL / min的流速从全血样品中分离出多达91％的靶细胞。此外，引入了WBC耗尽过程，该过程大大减少了WBC滞留在滤膜上的情况。进行了对捕获在膜上的细胞的免疫荧光分析测试，表明该设备可以在全血样品中提供可靠的CTC鉴定和CTC计数。最后，