Two-photon excited photodynamic therapy (TP-PDT) is not only able to offer deeper penetration depth but also much more precise 3D treatment than traditional one-photon excited PDT. However, the achievement of TP-PDT requires photosensitizers with large two-photon absorption cross sections, efficient generation of reactive oxygen species, and bright two-photon fluorescence. In this work, we present a simple AIE luminogen (AIEgen), IQ-TPA, with mitochondrial targeting and susceptible two-photon excitation for image-guided photodynamic therapy in cancer cells. This feasibility of utilizing small molecular multifunctional AIEgens for TP-PDT was demonstrated together with the merits of tiny size, good cell permeability, low dark cytotoxicity and easy synthesis, showing great potential for the development of future theranostic systems.

中文翻译：

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一种简单的靶向线粒体AIEgen的图像引导的双光子激发光动力疗法

与传统的单光子激发PDT相比，双光子激发光动力疗法（TP-PDT）不仅能够提供更深的穿透深度，而且还可以提供更精确的3D治疗。但是，TP-PDT的实现需要光敏剂具有较大的两光子吸收截面，有效生成活性氧物种以及明亮的两光子荧光。在这项工作中，我们提出了一种简单的AIE发光剂（AIEgen），IQ-TPA，具有线粒体靶向性和易感性的双光子激发，用于图像引导的光动力疗法治疗癌细胞。证明了利用小分子多功能AIEgens进行TP-PDT的可行性以及体积小，细胞通透性好，暗细胞毒性低和易于合成的优点，显示了开发未来治疗疗法系统的巨大潜力。