Engineering an (Ir)regular cytochrome P450: Mutations within the heme-binding pocket of a cytochrome P450 enabled the selective incorporation of an artificial Ir-porphyrin cofactor into the protein, in cells. This orthogonal metalloprotein showed enhanced behavior in unnatural carbene-mediated cyclopropanation of aliphatic and electron-deficient olefins.

中文翻译：

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非生物催化的人工金属酶的正交表达

工程化（Ir）常规细胞色素P450：细胞色素P450血红素结合口袋内的突变能够将人工Ir-卟啉辅因子选择性掺入细胞中的蛋白质中。这种正交的金属蛋白在非天然卡宾介导的脂肪族和缺电子烯烃的环丙烷化中表现出增强的行为。