The localization of liquid-ordered (L_o) and liquid-crystalline (L_α) phase domains on a silicon substrate with a microwell array is investigated. Although the phase separation of the L_o and L_α phases on both a giant unilamellar vesicle (GUV) and a supported membrane remains stable for a long time, the lateral diffusion of lipids across each domain boundary occurs quickly. Since the phase separation and domain arrangement are governed by the stiffness and lateral tension of the lipid membrane, the phase separation is rearranged on a micropatterned substrate. Similar phase separation of the L_o and L_α phases is observed at a lipid membrane suspended over a microwell. However, the L_α phase is preferred at a suspended membrane, and saturated lipids and cholesterol are excluded toward the supporting membrane on the periphery. Since the L_o domain area is reduced by anisotropic diffusion through the boundary between the suspended and supported membranes, a very slow reduction rate with a linear functional relation is observed. Finally, a localized L_α phase domain is observed at a membrane suspended over a microwell, which is surrounded by an L_o phase supported membrane.

中文翻译：

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悬浮在微孔上的脂质双层的液序/液晶相分离

液体有序（本地化大号_ö）和液晶（大号_α）与微孔阵列的硅衬底上的相区域进行了研究。虽然相分离大号_ö和大号_α在两个巨单层囊泡（GUV）和支撑膜相对于保持长时间稳定，在每个域边界脂质的横向扩散很快发生。由于相分离和畴排列受脂质膜的刚度和侧向张力支配，因此相分离被重新排列在微图案化的基底上。所述的类似相分离大号_ö和大号_α在悬浮在微孔上方的脂质膜上观察到液相。然而，大号_α相，优选在悬浮膜和饱和脂质和胆固醇被排除朝向上的外围支撑膜。由于各向异性扩散通过悬浮膜和支撑膜之间的边界使L _o区域面积减小，因此观察到非常缓慢的具有线性功能关系的还原速率。最后，局部大号_α相结构域在悬挂在微孔，这是由一个环绕的膜观察到大号_ö相支撑膜。