Hypochlorite (ClO^–) is one of the most important reactive oxygen species (ROS), which plays an important role in sustaining human innate immunity during microbial invasion. Moreover, ClO^– is a powerful oxidizer for water treatment. The safety of drinking water is closely related to its content. Herein, m-phenylenediamine (mPD) is used as a precursor to prepare carbon dots (named m-CDs) with highly fluorescent quantum yield (31.58% in water), and our investigation shows that the strong fluorescent emission of m-CDs can be effectively quenched by ClO^–. Based on these findings, we developed a novel fluorescent nanoprobe (m-CDs) for highly selective detection of ClO^–. The linear range was from 0.05 to 7 μM (R² = 0.998), and the limit of detection (S/N = 3) was as low as 0.012 μM. Moreover, a portable agarose hydrogel solid matrix-based ratiometric fluorescent nanoprobe (m-CDs@[Ru(bpy)₃]²⁺) sensor was subsequently developed for visual on-site detection of ClO^– with the naked eyes under a UV lamp, suggesting its potential in practical application with low cost and excellent performance in water quality monitoring. Additionally, intracellular detection of exogenous ClO^– was demonstrated via ratiometric imaging microscopy.

中文翻译：

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高效比例荧光纳米探针（m -CDs @ [Ru（bpy）₃ ] ²⁺）的制备，用于现场和活细胞中次氯酸盐的视觉和特异性检测

次氯酸盐（ClO ^–）是最重要的活性氧（ROS）之一，在微生物入侵过程中维持人类固有的免疫力中起着重要作用。此外，ClO ^–是用于水处理的强大氧化剂。饮用水的安全性与其含量密切相关。这里，米苯二胺（米PD）被用作前驱体，以制备碳点（命名为米与高荧光量子产率（在水中31.58％）-CdS），并且我们的调查显示的强荧光发射米-CdS可以被ClO ^–有效淬灭。基于这些发现，我们开发了一种新型的荧光纳米探针（m-CdS）为CLO的高度选择性地检测^- 。线性范围为0.05至7μM（R ² = 0.998），检测限（S / N = 3）低至0.012μM。此外，基于矩阵便携式琼脂糖凝胶固体比率荧光纳米探针（米-CdS @的[Ru（联吡啶）₃ ] ²⁺）传感器随后视觉现场检测CLO的开发^-在UV灯下用肉眼，暗示其在水成本监测中具有低成本和优异性能的实际应用潜力。此外，外源性CLO的细胞内检测^-通过比率成像显微镜证实。