Pep-1 is a cell-penetrating peptide that represents a powerful strategy for delivering large, hydrophilic therapeutic molecules into cells. Model membranes, such as lipid vesicles and planar bilayers, have been useful for investigating the direct translocation of cell-penetrating peptides. Here, we present a droplet interface bilayer-based approach to quantify pep-1-mediated β-galactosidase translocation. We found that β-galactosidase translocation is driven only by the negative transmembrane potential resulting from the asymmetric bilayers. The asymmetric droplet interface bilayer method may be generally applicable for high-throughput screening of the efficacy of cell-penetrating peptides.

中文翻译：

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膜不对称驱动的大分子货物的肽介导的膜运输。

Pep-1是一种细胞穿透性肽，代表了将大的亲水性治疗分子递送到细胞中的强大策略。模型膜，例如脂质囊泡和平面双层膜，已用于研究细胞穿透肽的直接易位。在这里，我们提出了一种基于液滴界面双层的方法来量化pep-1介导的β-半乳糖苷酶易位。我们发现，β-半乳糖苷酶易位仅由不对称双层产生的负跨膜电位驱动。非对称液滴界面双层方法通常可用于细胞穿透肽功效的高通量筛选。