In this work, we describe for the first time a genetic diagnosis platform employing a polydiallyldimethylammonium chloride (PDDA)-modified capillary and a liquid-based thermalization system for rapid, simple and quantitative DNA analysis with minimal user interaction. Positively charged PDDA is modified on the inner surface of the silicon dioxide capillary by using an electrostatic self-assembly approach that allows the negatively charged DNA to be separated from the lysate in less than 20 seconds. The capillary loaded with the PCR mix is incorporated in the thermalization system, which can achieve on-site real-time PCR. This system is based on the circulation of pre-heated liquids in the chamber, allowing for high-speed thermalization of the capillary and fast amplification. Multiple targets can be simultaneously analysed with multiplex spatial melting. Starting with live Escherichia coli (E. coli) cells in milk, as a realistic sample, the current method can achieve DNA extraction, amplification, and detection within 40 min.

中文翻译：

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毛细管实验室：一种快速，简单，定量的遗传分析平台，集核酸提取，扩增和检测于一体

在这项工作中，我们首次描述了使用聚二烯丙基二甲基氯化铵（PDDA）修饰的毛细管和基于液体的热化系统进行基因诊断的平台，该平台可进行快速，简单和定量的DNA分析，而用户交互作用最少。通过使用静电自组装方法，可在二氧化硅毛细管的内表面上修饰带正电荷的PDDA，该方法可使带负电荷的DNA在不到20秒的时间内从裂解物中分离出来。载有PCR混合物的毛细管被整合到热系统中，可以实现现场实时PCR。该系统基于腔室中预热液体的循环，可实现毛细管的高速热化和快速扩增。可以使用多重空间融合同时分析多个目标。从现场开始牛奶中的大肠杆菌（E. coli）细胞作为一种现实的样品，当前的方法可以在40分钟内实现DNA的提取，扩增和检测。