Missing link: Tetrafluoroterephthalonitrile (4F-2CN) was used to cyclize a model peptide, M2 macrophage-binding peptide, and in one step impart intrinsic fluorescence, improved serum stability, and enhanced target binding affinity. The intrinsic fluorescence is sufficient for monitoring biodistribution by whole-organ fluorescence imaging and cell internalization by flow cytometry.

中文翻译：

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简便的环化方法可提高肽血清的稳定性并赋予固有荧光

缺少的链接：四氟对苯二甲腈（4F-2CN）用于环化模型肽，M2巨噬细胞结合肽，并一步一步赋予内在荧光，改善的血清稳定性和增强的目标结合亲和力。固有荧光足以通过全器官荧光成像监测生物分布，并通过流式细胞术监测细胞内在化。