A novel electrochemiluminescence (ECL) sensor with composite consisted of silica-sol, Zinc oxide nanoparticles (ZnO NPs), polyvinylpyrrolidone (PVP) and tris(2, 2′-bipyridine) ruthenium (II) was constructed. A new method for simultaneous determination of quinapril hydrochloride (QHCl) and its metabolite quinaprilat hydrochloride (QTHCl) in human plasma was developed using the ECL sensor coupled with capillary electrophoresis (CE). ECL intensities of QHCl and QTHCl increased dramatically when the ECL sensor was used as working electrode. The running buffer contains 14 mmol/L phosphate (pH 8.0) and 20% n-propyl alcohol. Under optimized experimental conditions, the linearity ranges of the method are 0.007–8.0 μg/mL for QHCl and 0.009–8.3 μg/mL for QTHCl. The detection limits of QHCl and QTHCl (S/N=3) are 3.6 ng/mL and 3.9 ng/mL, respectively. The method was applied for the simultaneous determination of QHCl and QTHCl in human plasma with satisfactory results.

构造了一种新型的由硅溶胶，氧化锌纳米颗粒（ZnO NPs），聚乙烯吡咯烷酮（PVP）和三（2，2'-联吡啶）钌（II）组成的电化学发光（ECL）传感器。结合毛细管电泳（CE）的ECL传感器，开发了一种同时测定人血浆中盐酸奎纳普利（QHCl）及其代谢物盐酸奎那普利拉特（QTHCl）的新方法。当ECL传感器用作工作电极时，QHCl和QTHCl的ECL强度急剧增加。运行缓冲液包含14 mmol / L磷酸盐（pH 8.0）和20％正丙醇。在优化的实验条件下，该方法的线性范围对于QHCl为0.007–8.0μg/ mL，对于QTHCl为0.009–8.3μg/ mL。QHCl和QTHCl（S / N = 3）的检出限分别为3.6 ng / mL和3.9 ng / mL。