The formation of gold nanoparticle (Au NP)-loaded γ-polyglutamic acid (γ-PGA) nanogels (NGs) for computed tomography (CT) imaging of tumors is reported. γ-PGA with carboxyl groups activated by 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide hydrochloride is first emulsified to form NGs and then in situ chemically cross-linked with polyethylenimine (PEI)-entrapped Au NPs with partial polyethylene glycol (PEG) modification ([(Au⁰)₂₀₀–PEI·NH₂–mPEG]). The formed γ-PGA–[(Au⁰)₂₀₀–PEI·NH₂–mPEG] NGs with a size of 108.6 ± 19.1 nm display an X-ray attenuation property better than commercial iodinated small-molecular-contrast agents and can be uptaken by cancer cells more significantly than γ-PGA-stabilized single Au NPs at the same Au concentrations. These properties render the formed NGs with an ability to be used as an effective contrast agent for the CT imaging of cancer cells in vitro and a tumor model in vivo. The developed hybrid NGs may be promising for the CT imaging or theranostics of different biosystems.

中文翻译：

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负载金纳米粒子的γ-聚谷氨酸纳米凝胶的肿瘤计算机断层成像成像的便捷形成。

报道了用于肿瘤的计算机断层摄影（CT）成像的金纳米粒子（Au NP）负载的γ-聚谷氨酸（γ-PGA）纳米凝胶（NGs）的形成。首先将具有被1-乙基-3- [3-（二甲基氨基）丙基]碳二亚胺盐酸盐活化的羧基的γ-PGA乳化以形成NG，然后与包裹有聚乙烯亚胺（PEI）的Au NP与部分聚乙烯原位化学交联乙二醇（PEG）修饰（[[（Au ⁰）₂₀₀ –PEI·NH ₂ – m PEG]）。形成的γ- PGA– [（Au ⁰）₂₀₀ –PEI·NH ₂ – m尺寸为108.6±19.1 nm的PEG] NG的X射线衰减特性优于商业碘化的小分子造影剂，并且在相同的Au下比γ-PGA稳定的单个Au NP更能被癌细胞摄取。浓度。这些性质使得形成的NG具有用作体外癌细胞和体内肿瘤模型的CT成像的有效造影剂的能力。所开发的混合NG可能对不同生物系统的CT成像或诊断学有希望。