Preparation of high performance electrochemical biosensing interface for the sensitive and rapid detection of human metabolites is of great interest for health care and biomedical science. In this paper, based on the adhesion technique of marine mussels, we designed and prepared a novel biosensor with a micro/nano-biointerface of Fe₃O₄-Mn₃(PO₃)₂@Ni foam, which offered a three dimensional (3D) living environment for real cell. The constructed biosensor with a 3D micro/nano-biointerface of Fe₃O₄-Mn₃(PO₃)₂@Ni foam was characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and elemental mapping. Furthermore, the electrochemical experiments by electrochemical method for detection of superoxide anion (O₂^•−) in situ released by cells were carried out by this biosensor we proposed. Results indicated that the 3D interface of mussel-inspired Fe₃O₄-Mn₃(PO₃)₂@Ni foam offered an amicable platform for promoting cell adhesion, which was beneficial for enhancing biosensing activity. This proposed sensing platform provided high electroactivity and excellent electron transport with a lower detection limit (0.0170 μM), wider linear range 0.04–2.44 μM) and short diffusion distance to reaction sites. The case achieved the accurate detection of O₂^•− (in situ released by cells) based on the combination of mussel-inspired biomimetic adhesion technique, 3D micro/nano-biointerface construction and electrochemical biosensing technique.

制备用于人体代谢物的灵敏和快速检测的高性能电化学生物传感界面，对于医疗保健和生物医学领域非常重要。在本文中，我们基于海洋贻贝的粘附技术，设计并制备了一种新型的具有微型/纳米生物接口的Fe ₃ O ₄ -Mn ₃（PO ₃）₂ @Ni泡沫的生物传感器，该传感器可提供三维（ 3D）真实细胞的生存环境。具有Fe ₃ O ₄ -Mn ₃（PO ₃）₂的3D微/纳米生物界面的生物传感器通过扫描电子显微镜（SEM），能量色散谱（EDS）和元素图谱表征了@Ni泡沫。此外，我们提出了一种利用电化学方法检测细胞原位释放的超氧阴离子（O ₂ ^•-）的电化学实验。结果表明，贻贝启发的Fe ₃ O ₄ -Mn ₃（PO ₃）₂的3D界面镍泡沫为促进细胞粘附提供了友好的平台，这对于增强生物传感活性是有益的。该拟议的传感平台提供了高电活性和出色的电子传输性，具有较低的检测限（0.0170μM），更宽的线性范围（0.04–2.44μM）和到反应部位的短扩散距离。该案例基于贻贝启发式仿生粘附技术，3D微/纳米生物界面构建和电化学生物传感技术的结合，实现了对O ₂ ^•的精确检测（细胞原位释放）。