We demonstrate the quantitative spectroscopic characterization and imaging of biological tissue using coherent time-domain microscopy with a femtosecond resolution. We identify tissue constituents and perform dephasing time (T2) measurements of characteristic Raman active vibrations. This was shown in subcutaneous mouse fat embedded within collagen rich areas of the dermis and the muscle connective tissue. The demonstrated equivalent spectral resolution (<0.3 cm−1) is an order of magnitude better compared to commonly used frequency-domain methods for characterization of biological media. This provides with the important dimensions and parameters in biological media characterization and can become an effective tool in detecting minute changes in the bio-molecular composition and environment that is critical for molecular level diagnosis.

中文翻译：

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追踪生物组织中的分子去相

我们使用飞秒分辨率的相干时域显微镜展示了生物组织的定量光谱表征和成像。我们识别组织成分并对特征拉曼主动振动进行相移时间 (T2) 测量。这在嵌入真皮和肌肉结缔组织的富含胶原蛋白区域的皮下小鼠脂肪中得到证实。与常用的用于表征生物介质的频域方法相比，所证明的等效光谱分辨率（<0.3 cm-1）要好一个数量级。这提供了生物介质表征中的重要维度和参数，并且可以成为检测生物分子组成和环境中微小变化的有效工具，这对分子水平诊断至关重要。