The application of Surface Plasmon Resonance Imaging (SPRi) for the detection of transmembrane antigen of the Rhesus (Rh) blood group system is demonstrated. Clinically significant Rh blood group system antigens, including D, C, E, c, and e, can be simultaneously identified via solid phase immobilization assay, which offers significant time savings and assay simplification. Red blood cells (RBCs) flowed through the micro-channel, where a suitable condition for Rh blood group detection was an RBC dilution of 1:10 with a stop-flow condition. Stop flow showed an improvement in specific binding compared to continuous flow. Rh antigens required a longer incubation time to react with the immobilized antibody than A and B antigens due to the difference in antigen type and their location on the RBC. The interaction between the immobilized antibodies and their specific antigenic counterpart on the RBC showed a significant difference in RBC removal behavior using shear flow, measured from the decay of the SPR signal. The strength of the interaction between the immobilized antibody and RBC antigen was determined from the minimum wall shear stress required to start the decay process in the SPR signal. For a given range of immobilized antibody surface densities, the Rh antigen possesses a stronger interaction than A, B, and AB antigens. Identification of 82 samples of ABO and Rh blood groups using SPRi showed good agreement with the standard micro-column agglutination technique. A wider coverage of antigenic recognition for RBC when using the solid phase immobilization assay was demonstrated for the RBC with the antigenic site located on the transmembrane protein of the clinically significant Rh antigen. Given the level of accuracy and precision, the technique showed potential for the detection of the Rh minor blood group system.

证明了表面等离子体共振成像（SPRi）在恒河猴（Rh）血型系统跨膜抗原检测中的应用。可通过固相固定测定法同时鉴定具有临床意义的Rh血型系统抗原，包括D，C，E，c和e，这可节省大量时间并简化测定。红细胞（RBC）流经微通道，其中用于Rh血型检测的合适条件是在停止流动条件下将RBC稀释度为1:10。与连续流动相比，停止流动显示出特异性结合的改善。由于抗原类型和它们在RBC上的差异，Rh抗原需要比A和B抗原更长的孵育时间才能与固定化抗体反应。固定的抗体与其在RBC上的特异性抗原对应物之间的相互作用显示，使用剪切流从RPR信号的衰减测量，RBC去除行为存在显着差异。固定的抗体和RBC抗原之间相互作用的强度由开始SPR信号中的衰变过程所需的最小壁切应力确定。对于给定的固定抗体表面密度范围，Rh抗原比A，B和AB抗原具有更强的相互作用。使用SPRi鉴定的82个ABO和Rh血型样本与标准微柱凝集技术显示出良好的一致性。当使用固相固定测定法时，对于RBC而言，其抗原性位点位于具有临床意义的Rh抗原的跨膜蛋白上，证明了其对RBC的抗原识别具有更广泛的覆盖范围。考虑到准确度和精密度，该技术显示了检测Rh小血型系统的潜力。