Abstract Electrochemically reduced graphene oxide (ERGO) has attracted considerable interest in the electrochemical biosensing field. In this work, the π−π stacking assembly of phenoxyl-dextran (DexP) and sensitive electrochemical stripping analysis of gold nanoparticles (Au NPs) on the ERGO surface are conducted to develop a novel nonenzymatic glucose biosensing method. Concanavalin A (Con A) was covalently linked with Au NP to obtain a nanoprobe, which was used for the specific biorecognition of glucose at the ERGO/DexP biosensor. Based on the glucose-Con A-dextran competition reaction, the Au NP/Con A nanoprobes were quantitatively captured onto the biosensor surface. Through the electrochemical stripping analysis of Au NPs, sensitive signal transduction was achieved. ERGO not only enables the simple preparation of the biosensor but also improves the sensitivity of the method greatly. The high specificity of the Con A biorecognition and the relatively positive potential range for the gold stripping analysis exclude well the signal interferences involving in the conventional electrochemical glucose biosensors. Thus such a nonenzymatic glucose biosensing method featuring excellent performance, low cost and convenient signal transduction provides a great potential for the diabetes diagnosis application.

中文翻译：

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苯氧基-葡聚糖在电化学还原氧化石墨烯上的自组装用于葡萄糖的非酶生物传感

摘要电化学还原氧化石墨烯（ERGO）在电化学生物传感领域引起了相当大的兴趣。在这项工作中，苯氧基-葡聚糖 (DexP) 的 π-π 堆积组装和 ERGO 表面上金纳米粒子 (Au NPs) 的灵敏电化学剥离分析被用来开发一种新的非酶葡萄糖生物传感方法。Concanavalin A (Con A) 与 Au NP 共价连接以获得纳米探针，用于在 ERGO/DexP 生物传感器上对葡萄糖进行特异性生物识别。基于葡萄糖-Con A-葡聚糖竞争反应，Au NP/Con A 纳米探针被定量捕获到生物传感器表面。通过对Au NPs的电化学剥离分析，实现了灵敏的信号转导。ERGO 不仅使生物传感器的制备变得简单，而且大大提高了方法的灵敏度。Con A 生物识别的高特异性和金剥离分析的相对正电位范围很好地排除了传统电化学葡萄糖生物传感器中涉及的信号干扰。因此，这种具有优异性能、低成本和方便信号转导的非酶葡萄糖生物传感方法为糖尿病诊断应用提供了巨大的潜力。