A competitive assay between the antiviral Amantadine and the dye Thionine for the Cucurbit[8]uril cavity was carried out in a flow injection analysis system for the indirect fluorescence detection of Amantadine. Both, Cucurbit[7]uril and Cucurbit[8]uril Thionine complexes were evaluated for the competitive assay. The use of a 12-port injection valve allows the on-line reaction in the flow system. Once optimized all the experimental variables, the methodology developed allows the detection of Amantadine at the 0.16 µM level with excellent accuracy (Er ≤ 8.2%) and reproducibility (RSD ≤ 6.3%) for all the concentration range assayed. This one-step turn-on fluorescence methodology allows reaching sampling frequencies of 68 samples per hour. The selectivity of the method was evaluated against different antiviral drugs. Moreover, the performance of the methodology proposed was tested by the Amantadine determination in human serum and pharmaceutical formulations samples. The results demonstrated that the method can be applied to Amantadine determination in real samples of different nature with excellent recoveries, ranging from 83% to 98% depending on the matrix assayed.

在流动注射分析系统中对金刚烷胺的间接荧光检测进行了抗病毒金刚烷胺和染料硫氨酸对葫芦[8]脲腔的竞争测定。Cucurbit[7]uril 和 Cucurbit[8]uril 硫氨酸复合物均针对竞争性测定进行了评估。使用 12 通进样阀允许在流动系统中进行在线反应。一旦优化了所有实验变量，所开发的方法就可以在所有检测浓度范围内以出色的准确度 (Er ≤ 8.2%) 和重现性 (RSD ≤ 6.3%) 检测 0.16 µM 水平的金刚烷胺。这种一步开启荧光方法允许达到每小时 68 个样本的采样频率。该方法的选择性针对不同的抗病毒药物进行了评估。而且，所提出的方法的性能通过人血清和药物制剂样品中的金刚烷胺测定进行了测试。结果表明，该方法可用于不同性质的实际样品中金刚烷胺的测定，具有出色的回收率，取决于所测定的基质，回收率在 83% 至 98% 之间。