Crosslinked 2-bromoethyl methacrylate polymer (PBEMA) was prepared in micro-spherical form (2–5 µm) by precipitation polymerization methodology. The bromide substituent was substituted with an azide group, which was then coupled with 1-[(2-Propynyloxy)methyl]pyrene] via alkyne-azide click chemistry. The pyrene-linked microspheres showed an intense green-blue excimer emission with a maximum at 480 nm, implying π−π stacking between the pyrene moieties on the microsphere surfaces. This fluorescence emission is extremely sensitive to the aromatic nitro compounds. So that the green-blue light fades immediately upon addition of trace amounts of 2,4,6-trinitrotoluene (TNT), 2,4-dinitrotoluene (DNT) and 2,4,6-trinitro phenol (TNP) in 100% aqueous media. Stern-Volmer plots were employed for comparison of their fluorescence quenching effects. The plots revealed Stern-Volmer constants of 1.33 × 10⁵, 2.451 × 10⁵ and 1.076 × 10⁵ M⁻¹ for TNT, TNP and DNT, respectively. Furthermore, it has been observed that, the microspheres can be reused several times, without losing excimer emission properties.

通过沉淀聚合方法制备了微球形（2–5 µm）的交联的甲基丙烯酸2-溴乙基甲基酯聚合物（PBEMA）。溴取代基被叠氮基取代，然后通过炔叠氮点击化学。与pyr连接的微球显示出强烈的蓝绿色准分子发射，最大发射波长为480 nm，这意味着微球表面上的mo部分之间存在π-π堆积。这种荧光发射对芳族硝基化合物极为敏感。因此，在100％水溶液中添加痕量的2,4,6-三硝基甲苯（TNT），2,4-二硝基甲苯（DNT）和2,4,6-三硝基苯酚（TNP）后，绿蓝光立即消失媒体。使用斯特恩-沃尔默图比较它们的荧光猝灭效果。这些曲线显示的1.33×10的Stern-Volmer方程常数⁵，2.451×10 ⁵和1.076×10 ⁵ 中号^-1分别用于TNT，TNP和DNT。此外，已经观察到，微球可以重复使用几次，而不会损失准分子发射特性。