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Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice
Theranostics ( IF 12.4 ) Pub Date : 2017-07-08 , DOI: 10.7150/thno.18968
Jing Xia , Ying Zhang , Huanhuan Zhao , Jie Wang , Xueren Gao , Jinpeng Chen , Bo Fu , Yuqing Shen , Fengqin Miao , Jianqiong Zhang , Gaojun Teng

Ischemic stroke is one of the leading causes of morbidity and mortality worldwide. The expression of major histocompatibility complex class I (MHC-I) molecules in the central nervous system, which are silenced under normal physiological conditions, have been reported to be induced by injury stimulation. The purpose of this study was to determine whether MHC-I molecules could serve as molecular targets for the acute phase of ischemic stroke and to assess whether a high-affinity peptide specific for MHC-I molecules could be applied in the near-infrared imaging of cerebral ischemic mice. Quantitative real-time PCR and Western blotting were used to detect the expression of MHC-I molecules in two mouse models of cerebral ischemic stroke and an in vitro model of ischemia. The NetMHC 4.0 server was used to screen a high-affinity peptide specific for mouse MHC-I molecules. The Rosetta program was used to identify the specificity and affinity of the screened peptide (histocompatibility-2 binding peptide, H2BP). The results demonstrated that MHC-I molecules could serve as molecular targets for the acute phase of ischemic stroke. Cy5.5-H2BP molecular probes could be applied in the near-infrared imaging of cerebral ischemic mice. Research on the expression of MHC-I molecules in the acute phase after ischemia and MHC-I-targeted imaging may not only be helpful for understanding the mechanism of ischemic and hypoxic brain injury and repair but also has potential application value in the imaging of ischemic stroke.

中文翻译:

缺血性中风小鼠中枢神经系统MHC-I分子的非侵入性监测

缺血性中风是全球发病率和死亡率的主要原因之一。据报道,在正常生理条件下沉默的主要组织相容性复合体I类主要分子(MHC-1)在正常生理条件下沉默表达,是由损伤刺激引起的。这项研究的目的是确定MHC-I分子是否可以作为缺血性卒中急性期的分子靶标,并评估MHC-I分子特异的高亲和力肽是否可以用于MHC-I分子的近红外成像。脑缺血小鼠。实时荧光定量PCR和Western印迹法检测MHC-I分子在两种小鼠脑缺血性中风和体外模型中的表达缺血模型。NetMHC 4.0服务器用于筛选特异于小鼠MHC-1分子的高亲和力肽。使用Rosetta程序鉴定所筛选肽段(组织相容性2结合肽段,H2BP)的特异性和亲和力。结果表明,MHC-1分子可作为缺血性中风急性期的分子靶标。Cy5.5-H2BP分子探针可用于脑缺血小鼠的近红外成像。研究MHC-I分子在缺血后急性期的表达以及MHC-I靶向成像不仅有助于了解缺血性和缺氧性脑损伤和修复的机制,而且在缺血性成像中具有潜在的应用价值。中风。
更新日期:2017-11-01
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