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New Intracellular Shikimic Acid Biosensor for Monitoring Shikimate Synthesis in Corynebacterium glutamicum
ACS Synthetic Biology ( IF 4.7 ) Pub Date : 2017-11-15 00:00:00 , DOI: 10.1021/acssynbio.7b00339 Chang Liu 1, 2 , Bo Zhang 1, 3 , Yi-Ming Liu 1 , Ke-Qian Yang 1 , Shuang-Jiang Liu 1
ACS Synthetic Biology ( IF 4.7 ) Pub Date : 2017-11-15 00:00:00 , DOI: 10.1021/acssynbio.7b00339 Chang Liu 1, 2 , Bo Zhang 1, 3 , Yi-Ming Liu 1 , Ke-Qian Yang 1 , Shuang-Jiang Liu 1
Affiliation
The quantitative monitoring of intracellular metabolites with in vivo biosensors provides an efficient means of identifying high-yield strains and observing product accumulation in real time. In this study, a shikimic acid (SA) biosensor was constructed from a LysR-type transcriptional regulator (ShiR) of Corynebacterium glutamicum. The SA biosensor specifically responded to the increase of intracellular SA concentration over a linear range of 19.5 ± 3.6 to 120.9 ± 1.2 fmole at the single-cell level. This new SA biosensor was successfully used to (1) monitor the SA production of different C. glutamicum strains; (2) develop a novel result-oriented high-throughput ribosome binding site screening and sorting strategy that was used for engineering high-yield shikimate-producing strains; and (3) engineer a whole-cell biosensor through the coexpression of the SA sensor and a shikimate transporter shiA gene in C. glutamicum RES167. This work demonstrated that a new intracellular SA biosensor is a valuable tool facilitating the fast development of microbial SA producer.
更新日期：2017-11-15
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