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Optogenetics Manipulation Enables Prevention of Biofilm Formation of Engineered Pseudomonas aeruginosa on Surfaces
ACS Synthetic Biology ( IF 4.7 ) Pub Date : 2017-10-31 00:00:00 , DOI: 10.1021/acssynbio.7b00273
Lu Pu 1 , Shuai Yang 1 , Aiguo Xia 1 , Fan Jin 1, 2, 3
Affiliation  

Synthetic biologists have attempted to solve real-world problems, such as those of bacterial biofilms, that are involved in the pathogenesis of many clinical infections and difficult to eliminate. To address this, we employed a blue light responding system and integrated it into the chromosomes of Pseudomonas aeruginosa. With making rational adaptions and improvements of the light-activated system, we provided a robust and convenient means to spatiotemporally control gene expression and manipulate biological processes with minimal perturbation in P. aeruginosa. It increased the light-induced gene expression up to 20-fold. Moreover, we deliberately introduced a functional protein gene PA2133 containing an EAL domain to degrade c-di-GMP into the modified system, and showed that the optimally engineered optogenetic tool inhibited the formation of P. aeruginosa biofilms through the induction of blue light, resulting in much sparser and thinner biofilms. Our approach establishes a methodology for leveraging the tools of synthetic biology to guide biofilm formation and engineer biofilm patterns with unprecedented spatiotemporal resolution. Furthermore, the findings suggest that the synthetic optogenetic system may provide a promising strategy that could be applied to control and fight biofilms.

中文翻译:

光遗传学操纵能够防止工程化铜绿假单胞菌在表面上的生物膜形成。

合成生物学家已经尝试解决现实世界中的问题,例如细菌生物膜的问题,这些问题与许多临床感染的发病机理有关,并且难以消除。为了解决这个问题,我们采用了蓝光响应系统,并将其整合到铜绿假单胞菌的染色体中。通过对光激活系统进行合理的适应和改进,我们提供了一种鲁棒且方便的方法,以时空控制基因表达并以最小的扰动在铜绿假单胞菌中操纵生物过程。它使光诱导的基因表达增加了20倍。此外,我们特意引入了功能蛋白基因PA2133含有一个EAL结构域,以将c-di-GMP降解为修饰的系统,并表明经过优化设计的光遗传学工具通过诱导蓝光抑制了铜绿假单胞菌生物膜的形成,从而导致生物膜稀疏和薄。我们的方法建立了一种方法,可以利用合成生物学的工具来以前所未有的时空分辨率来指导生物膜的形成和工程化生物膜的模式。此外,研究结果表明,合成的光遗传系统可能提供可用于控制和对抗生物膜的有前途的策略。
更新日期:2017-10-31
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