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A High-Throughput Mass-Spectrometry-Based Assay for Identifying the Biochemical Functions of Putative Glycosidases
ChemBioChem ( IF 3.2 ) Pub Date : 2017-10-27 02:00:31 , DOI: 10.1002/cbic.201700292
Tianyuan Peng 1 , Gabe Nagy 1 , Jonathan C. Trinidad 1, 2 , Joy Marie Jackson 1 , Nicola L. B. Pohl 1
Affiliation  

Losing the right amount of weight: High-throughput identification of glycosidase biochemical functions, including those from hyperthermophiles, is achieved by observing a correct mass-loss from individual members of a thermostable library of potential sugar substrates and their corresponding products by using electrospray ionization mass spectrometry (ESI-MS).

中文翻译:

基于高通量质谱的分析方法,用于确定假定的糖苷酶的生化功能

减轻体重:通过使用电喷雾电离质谱法观察潜在糖底物及其相应产物的热稳定文库中各个成员的正确质量损失,从而实现对糖苷酶生化功能(包括超嗜热菌的功能)的高通量鉴定光谱法(ESI-MS)。
更新日期:2017-10-27
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