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Calcium Phosphate–Collagen Scaffold with Aligned Pore Channels for Enhanced Osteochondral Regeneration
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2017-10-27 , DOI: 10.1002/adhm.201700966
Yun-Jeong Seong 1 , In-Gu Kang 1 , Eun-Ho Song 1 , Hyoun-Ee Kim 1, 2 , Seol-Ha Jeong 1
Affiliation  

This study reports the development of a bilayered scaffold with aligned channels produced via a sequential coextrusion and unidirectional freezing process to facilitate upward bone‐marrow stem‐cell migration. The biomimetic scaffold with collagen and biphasic calcium phosphate (BCP) layers is successfully fabricated with matching of the cartilage and bone layers. The aligned structure results in an enhancement of the compressive strength, and the channels enable tight anchoring of the collagen layers on the BCP scaffolds compared with a randomly structured porous scaffold. An in vitro evaluation demonstrates that the aligned channels guide the cells to attach on the surface in highly stretched shapes and migrate upward faster than the random structure. In addition, in vivo assessment reveals that the aligned channels yield superior osteochondral tissue regeneration compared with the random structure. Moreover, the channel diameter greatly affects the tissue regeneration, and the scaffold with a channel diameter of ≈270 µm exhibits the optimal regeneration because of sufficient nutrient supply and adequate tissue ingrowth. These findings indicate that the introduction of aligned channels to a bilayered scaffold provides an effective approach for osteochondral tissue regeneration.

中文翻译:

磷酸钙-胶原支架具有对齐的孔道,可增强骨软骨再生

这项研究报告了通过顺序共挤出和单向冷冻过程产生的具有对齐通道的双层支架的开发,以促进骨髓干细胞向上迁移。具有胶原和双相磷酸钙(BCP)层的仿生支架可以成功地与软骨和骨层相匹配地制成。对齐的结构导致抗压强度的增强,并且与随机结构的多孔支架相比,通道能够将胶原层紧密锚固在BCP支架上。体外评估表明,对齐的通道可以引导细胞以高度拉伸的形状附着在表面上,并且比随机结构更快地向上迁移。此外,体内评估显示,与随机结构相比,对齐的通道可产生优异的骨软骨组织再生。此外,通道直径极大地影响了组织的再生,并且通道直径约为270 µm的支架由于营养供应充足和组织向内生长而表现出最佳的再生效果。这些发现表明,将对齐的通道引入双层支架为骨软骨组织再生提供了有效的方法。
更新日期:2017-10-27
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