Bisphenol A (BPA) is a highly toxic chemical, and its residue in milk product is threatening people's health due to its possible leaching from the packagings and cans with BPA coating. In this work, halides modified Au nanoparticles (NPs) as the modification substrates were first designed for rapid and sensitive determination of BPA residue in real milk by SERS method with the assistance of aggregation agents (Zn²⁺). It can be concluded that Au NPs modification substrate with assistance of the aggregation agent can remarkably improve the detection sensitivity of BPA residue, which can significantly enhance the SERS signal of BPA and achieve the trace-level detection of BPA residue. Under the optimal conditions, the limit of detection of BPA residue can be as low as to 4.3 × 10⁻⁹ mol/L (equal to 0.98 × 10⁻³ mg/kg), which is much less than the standard of European Union (0.6 mg/kg). And, there is a good linear relationship (R² = 0.990) between the intensity of SERS signal and the logarithm of BPA concentration in the range of 1.0 × 10⁻⁸–1.0 × 10⁻³ mol/L. By this method, the recovery of BPA residue ranges from 89.5% to 100.2% with relative standard deviation between 4.6% and 2.7%. The proposed SERS method proves to be reliable, highly sensitive and possesses good reproducibility, which is very promising for sensitive detection of bisphenols residue in foodstuff.

双酚A（BPA）是一种剧毒化学品，其在奶制品中的残留物可能危害人们的健康，因为它可能从带有BPA涂层的包装和罐中浸出。在这项工作中，首先设计了卤化物修饰的金纳米颗粒（NPs）作为修饰底物，用于在凝集剂（Zn ²⁺）辅助下通过SERS方法快速灵敏地测定真乳中的BPA残留。可以得出结论，在聚集剂的辅助下，Au NPs修饰底物可以显着提高BPA残基的检测灵敏度，可以显着增强BPA的SERS信号，实现痕量的BPA残基检测。在最佳条件下，BPA残留物的检出限可低至4.3×10 ^-9 mol / L（等于0.98×10 ^-3  mg / kg），远低于欧盟的标准（0.6 mg / kg）。并且，SERS信号的强度与BPA浓度的对数在1.0×10 ^-8 –1.0×10 ^-3  mol / L之间具有良好的线性关系（R ² = 0.990）。通过这种方法，BPA残留物的回收率为89.5％至100.2％，相对标准偏差为4.6％至2.7％。所提出的SERS方法被证明是可靠的，高度灵敏的并且具有良好的可重复性，这对于灵敏地检测食品中的双酚类残留物是非常有前途的。