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Surface sieving coordinated IMAC material for purification of His-tagged proteins
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2018-01-01 , DOI: 10.1016/j.aca.2017.10.021 Senwu Li , Kaiguang Yang , Lukuan Liu , Baofeng Zhao , Yuanbo Chen , Xiao Li , Lihua Zhang , Yukui Zhang
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2018-01-01 , DOI: 10.1016/j.aca.2017.10.021 Senwu Li , Kaiguang Yang , Lukuan Liu , Baofeng Zhao , Yuanbo Chen , Xiao Li , Lihua Zhang , Yukui Zhang
Tailor-made materials for the purification of proteins with His-tag was designed through synergizing the selectivity of surface sieving and metal ion affinity. By excluding impurity proteins out of the surface polymer network, such materials could purify His-tagged proteins from the crude cell lysis with purity up to 90%, improved by 14% compared to that obtained by the commercial metal chelating affinity materials. This study might promote the His-tagged protein purification to a new level.
中文翻译:
用于纯化 His 标签蛋白的表面筛分配位 IMAC 材料
通过协同表面筛分的选择性和金属离子亲和力,设计了用于纯化带有 His 标签的蛋白质的定制材料。通过将杂质蛋白质排除在表面聚合物网络之外,这种材料可以从粗细胞裂解液中纯化出带组氨酸标签的蛋白质,纯度高达 90%,与商业金属螯合亲和材料相比提高了 14%。这项研究可能将His-tagged蛋白纯化提升到一个新的水平。
更新日期:2018-01-01
中文翻译:
用于纯化 His 标签蛋白的表面筛分配位 IMAC 材料
通过协同表面筛分的选择性和金属离子亲和力,设计了用于纯化带有 His 标签的蛋白质的定制材料。通过将杂质蛋白质排除在表面聚合物网络之外,这种材料可以从粗细胞裂解液中纯化出带组氨酸标签的蛋白质,纯度高达 90%,与商业金属螯合亲和材料相比提高了 14%。这项研究可能将His-tagged蛋白纯化提升到一个新的水平。